Dissociated primary neurons () grown on microelectrode arrays () show several advantages which make them an optimal tool in drug discovery. The neurons in culture generate functional networks that exhibit electrically fingerprints comparable to their native brain tissue ().
NeuroProof routinely cultivates primary neurons from rodents for at least 28 days in vitro (DIV). This enables us to study maturation and developmental processes of neuronal network growth. With NeuroProof’s data analysis it is possible to resolve the developmental state with a precision of two days.
The spontaneous electrical activity of these cultures on a microelectrode array show a complex activity pattern, which is quantitatively described by more than 200 parameters by NeuroProof's mathematical approach. This pattern can be influenced by pharmaceuticals and nutraceuticals in a distinct and reproducible manner. This rich information content can be exploited for the phenotypic screening in drug discovery.
NeuroProof offers screening services using primary neuronal network cultures derived from
- frontal cortex
- amygdala/hippocampus co-culture
- midbrain/cortex co-culture
- spinal cord
- or human neuronal cultures derived from induced pluripotent stem cells.
Primary tissue cultures are dissected between embryonic day E14 and E18 according to the tissue. Please contact us for more information.